Photo by Sven Mieke on Unsplash

Normalizing single cell RNA sequencing data — Pitfalls and Recommendations

The goals of a single cell RNA sequencing (scRNA-seq) project are often Identification of subpopulations and Differential Gene Expression Analysis. To avoid the ‘curse of dimensionality’, Highly Variable Genes (HVGs) are used for cluster analysis. Several studies have shown that selection of HVGs is sensitive to the choice of method used for normalization of raw…

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Shivangi Patel

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Biologist, aspiring Data Scientist

Towards Data Science

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